| Director | Joe Dante |
|---|---|
| Budget | $80 million |
| Box office | $68.5 million |
Weary of playing antagonist roles in Bugs Bunny's films, Daffy Duck demands his own animated film, only to be fired by Warner Bros.' Vice President of Comedy, Kate Houghton. Security guard and aspiring stuntman DJ Drake is ordered to escort Daffy from the studio, but Daffy escapes and the water tower is destroyed in the ensuing chase, resulting in DJ's firing. Daffy follows DJ home, where the latter receives a message from his father, actor Damian Drake, whom DJ learns is a secret agent. Damian tells DJ to travel to Las Vegas, contact his associate Dusty Tails and find the "Blue Monkey" diamond before he gets captured by the Acme Corporation, led by Mr. Chairman. DJ and Daffy depart for Las Vegas.
Bugs' routines fail without Daffy, so Kate is ordered to rehire Daffy or face termination herself. Kate and Bugs arrive at DJ's house, where they find and use Damian's TVR Tuscan in pursuit of DJ and Daffy. In Las Vegas, DJ and Daffy meet Dusty in a casino owned by Yosemite Sam, who is employed by Acme. Dusty gives them a unique playing card, which provides a clue to finding the diamond, before Sam and his henchmen, Nasty Canasta and Cottontail Smith, steal Jeff Gordon's car to pursue DJ and Daffy for the card, but they escape with Bugs and Kate aboard the Tuscan taking flight. When the Tuscan crashes in a desert, the group camps out for the night while Bugs fails to convince Daffy to take his job back, with the latter revealing he envies Bugs for being so popular with so little effort. The next day, after the group finds Walmart, Wile E. Coyote tries in vain to steal the card from the group.
The group eventually discovers Area 52, run by a woman known as Mother, who shows them a short film about the Blue Monkey, which can devolve humans into monkeys. She then equips the group with spy gadgets for their mission. Marvin the Martian, imprisoned in the facility, leads a group of other imprisoned aliens to try to steal the card, but the group escapes. Seeing the Mona Lisa's face on the card, the group conclude they must view the painting in the Louvre in Paris.
At the Louvre, the group discover that the card contains a viewing window for the Mona Lisa and use it to discover a hidden map of Africa. Elmer Fudd appears and chases Bugs and Daffy through several paintings to obtain the card. Meanwhile, Kate is kidnapped by Beaky Buzzard and Smith, Mr. Chairman's bodyguard. DJ rescues Kate, while Elmer disintegrates into tiny dots after emerging from a pointillist painting.
The group travels to Africa and meet Granny, Sylvester and Tweety, who escort them to the ruins of a jungle temple containing the Blue Monkey. There, Granny and company reveal themselves to be Mr. Chairman, Smith and Taz in disguise. Mr. Chairman teleports everyone to Acme and tricks DJ into giving him the diamond in exchange for Damian's release, with him going back on his word once the diamond is in his hands.
Marvin is sent to place the diamond in an Acme satellite's ray gun; with it, Mr. Chairman plans to turn everyone on the planet but himself into monkeys to make his products before reversing the effects so they can purchase them. DJ and Kate face off against a robotic dog and rescue Damian from one of Wile E. Coyote's death traps, while Bugs and Daffy chase Marvin into space. Marvin battles Bugs while Daffy becomes Duck Dodgers to stop the ray gun. Bugs manages to fight back and defeats Marvin by plugging his finger into his gun, imprisoning him inside his own bubble. Daffy uses his own bill to compress the ray gun's blast into two smaller ones through his nostrils. One of them reaches Earth and transforms Mr. Chairman into a monkey, leading to his arrest. Bugs and Daffy return to Earth, where it is revealed that the preceding events were staged. Bugs promises Daffy they will be equal partners, just before the latter is flattened by the Looney Tunes rings. Porky Pig attempts to close the film with "That's all, folks!", but the studio closes before he could say it, much to his indignation.